Journal: Cancer Medicine

Article Title: CCNG1 (Cyclin G1) regulation by mutant‐P53 via induction of Notch3 expression promotes high‐grade serous ovarian cancer (HGSOC) tumorigenesis and progression

doi: 10.1002/cam4.1812

Figure Lengend Snippet: Effects of down‐regulating CCNG1 on the migration and invasion ability of ovarian cancer cell lines. The number of migrating and invasion cells in silenced CCNG1group was significantly reduced in A2780 (A) or HO8910 (B). The numerical values were mean ± SD of three replicates. All the experiments were repeated three times using the same batch of cells

Article Snippet: Human ovarian cancer cell line A2780 (Procell, China, CL‐0013) and HO8910 (Procell, China, CL‐0113) were cultured in RPMI 1640 culture medium and SKOV3 (ATCC, USA, HBT‐77) in McCoy's 5A medium, supplemented with 10% fetal bovine serum (FBS, Gibco).

Techniques: Migration

Journal: Cancer Medicine

Article Title: CCNG1 (Cyclin G1) regulation by mutant‐P53 via induction of Notch3 expression promotes high‐grade serous ovarian cancer (HGSOC) tumorigenesis and progression

doi: 10.1002/cam4.1812

Figure Lengend Snippet: CCNG1 modulated cisplatin sensitivity. A, After exposure to cisplatin, CCNG1 protein expression in ovarian cancer cells was time‐dependently up‐regulated. B and C, Cell inhibitory rates and IC50 of cisplatin in HO8910 and A2780 were significantly changed after down‐regulation of CCNG1 expression by shRNA. Cell viability detection, from cells seeded in 96‐well plates to calculate IC50, was repeated three times to obtain three IC50

Article Snippet: Human ovarian cancer cell line A2780 (Procell, China, CL‐0013) and HO8910 (Procell, China, CL‐0113) were cultured in RPMI 1640 culture medium and SKOV3 (ATCC, USA, HBT‐77) in McCoy's 5A medium, supplemented with 10% fetal bovine serum (FBS, Gibco).

Techniques: Expressing, shRNA

Journal: Cancer Medicine

Article Title: CCNG1 (Cyclin G1) regulation by mutant‐P53 via induction of Notch3 expression promotes high‐grade serous ovarian cancer (HGSOC) tumorigenesis and progression

doi: 10.1002/cam4.1812

Figure Lengend Snippet: Down‐regulation of CCNG1 reduced tumor metastasis in vivo . A, H&E staining of lung metastasis of shCCNG1‐A2780 cell line and its control. B, Nude mice injected with shCCNG1‐A2780 cell line developed fewer lung metastatic foci than its control ( P < 0.0001). C, CCNG1 was regulated by P53mt via induction of Notch3 expression, which ultimately promotes both HGSOC tumor cell metastasis and cisplatin resistance

Article Snippet: Human ovarian cancer cell line A2780 (Procell, China, CL‐0013) and HO8910 (Procell, China, CL‐0113) were cultured in RPMI 1640 culture medium and SKOV3 (ATCC, USA, HBT‐77) in McCoy's 5A medium, supplemented with 10% fetal bovine serum (FBS, Gibco).

Techniques: In Vivo, Staining, Control, Injection, Expressing

Journal: Oncology Reports

Article Title: Gomisin A enhances the antitumor effect of paclitaxel by suppressing oxidative stress in ovarian cancer

doi: 10.3892/or.2022.8417

Figure Lengend Snippet: GA enhances the proliferation-inhibiting effect of PTX on ovarian cancer cells. (A) Structure of GA. (B) Inhibitory effects of different treatments on SKOV3 and A2780 cells. (C) Colony formation assay of cell clonality of SKOV3 and A2780 cells. (D) Flow cytometric and statistical analysis of apoptosis. (E) Flow cytometric and statistical analysis of cell cycle progression. Data are expressed as the mean ± SD from three independent experiments. ** P<0.01 vs. Ctrl; # P<0.05, ## P<0.01 vs. PTX. Ctr, control; GA, Gomisin A; NAC, N-acetyl cysteine; PTX, paclitaxel.

Article Snippet: SKOV3 and A2780 cell lines were obtained from the Prostate Disease Prevention and Control Centre, Basic Medical College of Jilin University; the SKOV3 (cat. no. CL-0215) and A2780 (cat. no. CL-0013) cell lines were originally purchased from Procell Life Science & Technology Co., Ltd.

Techniques: Colony Assay, Control

Journal: Oncology Reports

Article Title: Gomisin A enhances the antitumor effect of paclitaxel by suppressing oxidative stress in ovarian cancer

doi: 10.3892/or.2022.8417

Figure Lengend Snippet: GA enhances the inhibitory effect of PTX on ovarian cancer cell migration and invasion. (A and C) Migratory ability of SKOV3 and A2780 cells following different treatments, as determined by the wound scratch assay. (B and D) Invasive ability of SKOV3 and A2780 cells following different treatments, as determined by Transwell assay. (E) Expression levels of MMP-2, as detected by western blot analysis. Data are expressed as the mean ± SD from three independent experiments. * P<0.05, ** P<0.01 vs. Ctrl; # P<0.05, ## P<0.01 vs. PTX. Ctr, control; GA, Gomisin A; NAC, N-acetyl cysteine; PTX, paclitaxel; MMP-2, matrix metallopeptidase 2.

Article Snippet: SKOV3 and A2780 cell lines were obtained from the Prostate Disease Prevention and Control Centre, Basic Medical College of Jilin University; the SKOV3 (cat. no. CL-0215) and A2780 (cat. no. CL-0013) cell lines were originally purchased from Procell Life Science & Technology Co., Ltd.

Techniques: Migration, Wound Healing Assay, Transwell Assay, Expressing, Western Blot, Control

Journal: Oncology Reports

Article Title: Gomisin A enhances the antitumor effect of paclitaxel by suppressing oxidative stress in ovarian cancer

doi: 10.3892/or.2022.8417

Figure Lengend Snippet: GA enhances the inhibitory effect of PTX by downregulating the levels of ROS. (A) DHE staining and statistical analysis of ROS in SKOV3 and A2780 cells following different treatments. (B and C) Flow cytometric and statistical analysis of ROS levels in SKOV3 and A2780 cells following different treatments, as determined by DCHF-DA staining. (D) Expression levels of Keap-1, Nrf-2 and NQO1, as detected by western blot analysis. Data are expressed as the mean ± SD from three independent experiments. * P<0.05, ** P<0.01 vs. Ctrl; # P<0.05, ## P<0.01 vs. PTX. Ctr, control; DHE, dihydroethidium; GA, Gomisin A; NAC, N-acetyl cysteine; PTX, paclitaxel; ROS, reactive oxygen species.

Article Snippet: SKOV3 and A2780 cell lines were obtained from the Prostate Disease Prevention and Control Centre, Basic Medical College of Jilin University; the SKOV3 (cat. no. CL-0215) and A2780 (cat. no. CL-0013) cell lines were originally purchased from Procell Life Science & Technology Co., Ltd.

Techniques: Staining, Expressing, Western Blot, Control